COUPLAGE ET FRAGMENTATION D’ANTICORPS
Proteogenix propose des services de couplage d’anticorps à différents fluochromes, enzymes et molécules :
- Horseradish Peroxydase (HRP)
- Phosphatase Alcaline
- Biotine
- KLH
- BSA
Nous couplons aussi vos anticorps à des colorants et fluorophores / fluorochromes :
FITCEXCITATION (LASER) : 488 nm(Blue Diode / HeAr laser)EMISSION : 525 nmNOTE : Fluorescein isothioyanate; the most commonly used fluorochrome for FACS analysis detected in FL1 channel
Dyomics 547 EXCITATION (LASER) : 557 nm (Yellow laser)EMISSION : 574 nmNOTE : Alternative to Cy3 and Alexa Fluor® 535
PE EXCITATION (LASER) : 488 nm (Blue Diode / HeAr laser)EMISSION : 575 nmNOTE : R-Phycoerythrin; PE is a large protein (240 kDa) containing 25 fluorophores; PE is the most commonly used dye for FACS analysis detected in FL2 channel on most instruments;suitable for detection of low density antigens
PE-Dyomics 590EXCITATION (LASER) : 488 nm (Blue Diode / HeAr laser)EMISSION : 599 nmNOTE : R-Phycoerythrin - Dyomics 590; tandem dye of PE and Dyomics 590, same spectral properties as PE-Texas Red (ECD) tandem dye; detected in FL3 channel on single laser instruments
PE-Dyomics 647 EXCITATION (LASER) : 488 nm (Blue Diode / HeAr laser)EMISSION : 672 nmNOTE : R-Phycoerythrin - Dyomics 647; tandem dye of PE and Dyomics 647 detected in FL3 or FL4 on most instrumentssimilar to PE-Cy5 (PC5) and PE-Alexa Fluor® 647
APCEXCITATION (LASER) : 633 nm (Red laser Red Diode / HeNe laser)EMISSION : 660 nmNOTE : Allophycocyanin; APC is a large protein (105 kDa) containing many fluorophores; APC is emerging as one of the brightest fluorescent probes for FACS analysis; detected in FL4 on dual laser instruments
Dyomics 647EXCITATION (LASER) : 633 nm (Red laser, Red Diode / HeNe laser)EMISSION : 672 nmNOTE : Dyomics 647; DY647 is pentamethine based small fluorochrome detected in FL4 on dual laser instruments;except for FACS analysis, where it may replace use of traditional APC (namely good choice for intracellular staining), it is excellent for microscopy and microarrays;similar to Cy5 and Alexa Fluor® 647
Si vous ne trouvez pas la molécule à laquelle vous souhaitez coupler votre anticorps, contactez-nous, nous avons peut-être déjà réalisé des couplages du même type.
Nous proposons de fragmenter vos anticorps par digestion/traitement par :
- la pepsine pour obtenir des fragments Fab’2
ou
- la papaïne pour obtenir des fragments Fab
Prestations complémentaires disponibles :